ARTICLE · ROOT ZONE & RHIZOSPHERE

Bacillus in the root zone: why strain and application matter

7 min read

Dense network of fine roots running through dark soil

A crop can only perform well when the environment around its roots is in good shape. Water, oxygen, nutrition, pH and root temperature all play an important part. But that same root zone is also home to microorganisms that can influence the processes around the root.

That is why bacteria from the genus Bacillus are increasingly applied in a targeted way in professional growing systems. Not because every Bacillus bacterium automatically has a positive effect, but because carefully selected strains can have useful properties. Understanding Bacillus in the root zone starts with that distinction.

The result, however, isn’t determined only by the bacterium in the package. The chosen strain, formulation, rate, storage conditions and application method must suit the crop and the growing system. And after application, the microorganism has to actually reach the root zone and become active under the conditions it finds there.

Bacillus is not one single bacterium

Bacillus is often talked about as if it were one bacterium with one fixed effect. In reality, it is a large genus with many species and an even larger number of strains.

A strain is a specific variant within a species. Many properties are determined at exactly that level. Two strains with the same species name can behave differently in the root zone, produce different compounds and become active under different conditions.

It’s comparable to crop varieties. Two tomato varieties are both tomatoes, yet they can differ widely in growth, yield, susceptibility and suitability for a particular growing system. The same applies to microorganisms: the genus name alone doesn’t tell you what a strain can do in practice.

So “it contains Bacillus” is not, on its own, a good basis for judging a product. More important questions are:

  • Which strains does the product contain?
  • Why were these particular strains selected?
  • Are they stable enough in the formulation?
  • What application is the product intended for?
  • Under which conditions can the strains become active?
  • How should the product be stored and applied?

Why Bacillus is interesting for microbial products

An important property of several Bacillus strains is that they can form endospores. A spore is a dormant form that allows the bacterium to survive less favorable conditions.

That is valuable when producing, storing and applying a microbial formulation. Microorganisms are, after all, living organisms. They need to survive the period between production and use without losing their viability.

Spore formation can help. It generally makes certain Bacillus strains more resistant to drying out and temporary environmental stress than bacteria that don’t form spores.

Still, there is an important difference between surviving and functioning. A spore that has survived in the package still has to, after application:

  1. end up in the root zone;
  2. germinate under the conditions present;
  3. adapt to its environment;
  4. make contact with the root surface or its immediate surroundings;
  5. become active enough to make a relevant contribution.

Spore formation is therefore a practical advantage, but not a full explanation of how a product works.

Where the interaction with the plant takes place

The soil, substrate or water directly around the roots is called the rhizosphere: the narrow zone where roots, microorganisms, nutrients, organic compounds, water and oxygen all influence each other.

Roots release sugars, amino acids and other compounds. These root exudates are a food source for certain microorganisms. At the same time, microorganisms can produce compounds or carry out conversions that affect the immediate root environment.

Young plants in a propagation tray with roots visibly growing through the cells

The root surface itself is called the rhizoplane. Some bacterial strains can settle there temporarily or for longer. Others are found mainly in the material just around the root.

That colonization doesn’t happen automatically. The root zone is not an empty space where an added microorganism has free rein. There is already an existing microbial community, and moisture, temperature, nutrition, pH and oxygen change all the time.

A selected strain has to be able to hold its own within that reality. That is why laboratory results alone are not enough. Formulation and application under practical conditions count as well.

Supporting nutrient processes

Plants can only take up nutrients when these are present in an available form and in the right place around the root. In soil and substrate, nutrients can be temporarily tied up, chemically bound or become part of organic matter.

Certain Bacillus strains can produce enzymes, organic acids or other metabolites that play a role in conversion processes in the root zone. Research describes effects on, among other things, the dynamics of phosphate and certain trace elements.

That doesn’t mean a bacterium replaces fertilizers or can release nutrients without limit. The available supply, pH, moisture, oxygen supply and chemical composition of the root environment remain decisive.

A microbial application should therefore be seen as part of the overall crop program. Fertilization, irrigation and root zone management remain essential. Selected microorganisms can support these processes, but they don’t correct a fundamentally wrong feeding schedule.

So the practical question is not: “Can this bacterium make nutrients?” The better question is:

Can this strain, under the conditions of my crop, help processes around the available nutrients and the root run more favorably?

Root development and a more uniform crop

The root is the link between the plant and its growing environment. The size, branching and activity of the root system determine how much water and nutrients a plant can reach.

Certain Bacillus strains are known to produce compounds involved in the interaction with plants and roots. Research describes, among other things, changes in root branching and root development.

Here too, the final result depends on the strain and the growing conditions. A bacterium cannot guarantee a healthy root system when the root zone is structurally too wet, low in oxygen, too cold or chemically out of balance.

The commercial value of a targeted microbial application lies mainly in its ability to support root zone management. When the application fits the crop well, it can contribute to a more active root environment and a more uniform crop.

“More roots” is not always the only or best measure. Root color, branching, distribution and activity matter too.

Support under stressful conditions

In practice, crops regularly face abiotic stress. Think of:

  • high or low root temperatures;
  • fluctuations in moisture;
  • increased salt load;
  • temporary lack of oxygen;
  • large changes in light or evaporation;
  • variation in the composition of the nutrient solution.

Scientific research (opens in a new tab) has described Bacillus strains that can influence processes linked to how plants respond to such conditions, for example changes in signaling compounds, antioxidant processes and root development.

That doesn’t mean a microbial application prevents stress. A plant without enough water will still suffer from drought, and a root zone low in oxygen needs a technical fix.

A suitable microbial application can, however, be part of a broader program aimed at helping the crop and root zone function better when conditions change.

Exact claims must always match the strain studied, the product concerned and its registration in the market where it is used.

Why strain selection matters more than a long ingredient list

A product with many different microorganisms is not automatically better than one with a limited number of carefully chosen strains.

Strains in a formulation need to be more than interesting on their own. They also have to:

  • remain stable alongside each other;
  • suit the chosen carrier and formulation;
  • be able to become active in the intended root environment;
  • not work against each other unnecessarily;
  • reach the application alive and in sufficient numbers.

That is why a long list of species says little when it isn’t clear why the organisms were selected and how the product should be used.

At BIOMICROBES, we prefer to look at the function of the selected strains and their place in the growing system. The aim is not to put as many names as possible on a label, but to make a microbial solution technically workable.

Conditions differ by growing system

An application in soil is not the same as one in substrate or hydroponics.

Grower crouching on a tilled field while checking a tablet

Soil-grown crops

Soil usually contains a large and diverse microbial community. At the same time, pH, soil structure, moisture, temperature and organic matter can vary widely within a single field.

An added microorganism therefore enters a complex and changing environment. A good application calls for attention to soil condition, root activity, timing and available moisture. Read more about open-field growing.

Substrate cultivation

Substrates such as stone wool, coir and peat each have their own physical and chemical properties. Buffering capacity, moisture distribution and the amount of organic material also differ.

In a substrate system, the grower can steer the root zone precisely, but changes can also happen quickly. Rate and frequency must therefore match the irrigation schedule, substrate volume and drain strategy. Read more about greenhouse and substrate cultivation.

Hydroponics

In hydroponic systems, microorganisms are directly affected by water temperature, oxygen, nutrients, circulation and the system’s hygiene strategy.

Cleaning is important, but a strong disinfection step can also damage the microorganisms you have deliberately added. That is why microbial application and water treatment should be planned as one program.

Read more about this on our water microbiology page.

Watch the combination with cleaning products

A common mistake is applying living microbial products at the same time as products designed to kill microorganisms.

Oxidizing agents such as hydrogen peroxide and peracetic acid are used to clean lines, water and equipment. That can be an important part of system hygiene. At the same time, these products usually don’t distinguish between unwanted microorganisms and those you have deliberately added.

That is why a microbial application and a strong cleaning or disinfection step generally don’t belong in the same tank or at the same time.

The right interval depends on, among other things:

  • the cleaning product used;
  • its concentration;
  • contact time;
  • the irrigation system;
  • the amount of residual product;
  • the application advice for the microbial product.

Always follow the current label and have the combination technically assessed when in doubt. The wrong mixing moment can make a good microbial product largely ineffective before it even reaches the root.

What can you monitor in practice?

The effect of microbiology can’t always be reduced to one direct, visible change. It’s wise to decide before application what you want to improve and which data you will follow.

For example:

  • root color and root branching;
  • uniformity within the crop;
  • development of young plants;
  • EC and pH in feed and drain;
  • irrigation volume and drain percentage;
  • crop response during stress periods;
  • losses or lagging plants;
  • yield and product quality over a longer period.

Preferably work with a clear reference or a defined trial area. Don’t change many other parts of the crop program at the same time during the assessment, or it will be hard to tell afterwards what caused a difference.

Also, don’t expect every application to show visible results within a fixed number of days. Microbial processes depend on crop stage, root activity and conditions in the system. General promises such as “visible results within seven days” are therefore not careful without product-specific support.

What Bacillus is not

A microbial solution based on Bacillus is not a replacement for a good fertilization, irrigation or hygiene plan.

Nor is every Bacillus product automatically a fertilizer, biostimulant or crop protection product. The legal product category and permitted claims differ by product and by market. Always use a product according to the current label and the local authorization or registration.

A carefully formulated product can, however, be a targeted tool within professional root zone management.

In the end, the difference lies not only in which bacterium is named, but in the whole chain:

strain selection → production → formulation → storage → application → conditions in the root zone

If one of these links isn’t right, it can limit the final performance.

SELECTED STRAINS IN PRACTICE

The role of BIOMICROBES 08

BIOMICROBES 08 is our microbial solution based on carefully selected Bacillus strains.

The product was developed for targeted application in the root zone. We look not only at the microorganism itself, but also at how it is applied within the existing crop program.

For responsible advice, we therefore want to know:

  • which crop is being grown;
  • in which growing system;
  • which substrate or soil is used;
  • how water and nutrients are delivered;
  • which cleaning and disinfection products are used;
  • which practical crop goal is the priority.

Based on that, we can assess where a microbial application fits in the program and which points need attention.

Want to explore whether selected Bacillus strains could add value in your growing system? Get in touch with BIOMICROBES. We’ll look at the application from the perspective of the crop, the root zone and the technical setup of the system.

Frequently asked questions

Is every Bacillus bacterium beneficial for plants?

No. Bacillus is a large genus with many species and strains, and their properties vary widely. For a targeted application, strain selection, quality control and formulation are important.

Can Bacillus replace fertilizer?

No. Microorganisms don’t create unlimited new nutrients. Certain strains can be involved in conversion processes around the nutrients that are present. A suitable fertilization plan remains essential.

How quickly can I expect results?

There is no general timeframe. It depends on the strain, formulation, rate, root activity, crop stage and conditions in the root zone. Assess the application over a suitable period, based on measurement points chosen in advance.

Does Bacillus work the same way in every growing system?

No. Soil, substrate and hydroponics each have different conditions. The application must be matched to, among other things, the root environment, irrigation strategy, water quality and system hygiene.

Is a product with more strains automatically better?

No. The number of strains says little without information about their properties, how they combine, their stability and their suitability for the intended use. A smaller, purpose-built composition can be technically stronger than a long ingredient list.

Sources and further reading

  1. European Union. Regulation (EU) 2019/1009 on EU fertilising products.
    EUR-Lex: Regulation (EU) 2019/1009 (opens in a new tab)
  2. Miljaković, D., Marinković, J. & Balešević-Tubić, S. (2020). The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms.
    https://doi.org/10.3390/microorganisms8050705 (opens in a new tab)
  3. Hashem, A. et al. (2019). Bacillus subtilis: a plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences.
    https://doi.org/10.1016/j.sjbs.2019.05.004 (opens in a new tab)
  4. Frontiers in Plant Science (2021). Plant-associated rhizobacteria for biocontrol and plant growth enhancement.
    frontiersin.org: 10.3389/fpls.2021.634796 (opens in a new tab)
  5. Plants (2022). Bacillus for plant growth promotion and stress resilience: what have we learned?
    mdpi.com: Plants 11(19), 2482 (opens in a new tab)